The purpose of this research was to study the purification process of flavonoids in Malus toringoides (Rehd.) Hughes leaves (FMTs), and the purification parameters were optimised by adsorption and desorption tests. AB-8 resin was selected by comparing the adsorption and desorption ability of FMTs on twelve types of resins. The results showed that the optimal purification parameters of static adsorption and desorption were the sample at pH=4 with 3 h of adsorption; the desorption solvent was 70 % ethanol (pH = 7.0) with 3 h of desorption. The best dynamic parameters were a sample loading amount of 9 bed volumes (BV) at a feeding flow rate of 2 BV h-1 and washing the column with 6 BV of water, followed by subsequent flushing with 20 % (6 BV), 40 % (6 BV), 60 % (6 BV) and 80 % (6 BV) ethanol in water (v/v) at an elution flow rate of 2 BV h-1. Under these conditions, the purity of FMTs increased from 32.79% to 59.18%. The existence of flavonoids was confirmed by their characteristic functional groups that were identified by infrared spectroscopy. Meanwhile, FMTs in the dosage range of 50 to 400 ?g mL-1 showed a significant anti-inflammatory effect on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophage cells, which could inhibit the secretion of NO, IL-6, IL-1β and TNF-α through the inhibition of inflammatory-related gene expression.