In recent years, the wide application of organic pesticides caused serious contaminations of water, soil, and atmospheric. The development of fast, high-efficiency, and convenient determination of organic pesticides has become a significant research topic in the field of environmental science. In comparison to traditional detecting techniques of organic pesticides, determination of organic pesticides by fluorescent probes has advantages of high detection sensitivity, strong stability, and easy to handle. A full survey on the determination of organic pesticides by fluorescent probes of small organic molecules, supermolecules, and metal-organic frameworks (MOFs) have been reported in this review, respectively. And the conrresponding types of fluorescent probes, varieties of organic pesticides and solvents, reaction phenom-ena, and detection mechanisms have been discussed. Finally, the development tendency of MOFs fluores-cent probes in the determination of organic pesticides was summarized and prospected emphatically. In the future, the determination of organic pesticides will continue the tendency of exploring fast, efficient, and environmental friendly detecting techniques, and develop hyphenated techniques of organic pesticides detecting and pollution restoration in China.