Polyhexamethyleneguanidine hydrochloride (PHMG) was prepared by melt polycondensation of ethylenediamine (GHC) and guanidine hydrochloride (GHC). Then double bond functionalized PHMG (MPHMG) was prepared by reacting PHMG with glycidyl methacrylate (GMA)PHMG and the successful synthesis of MPHMG was characterized by FTIR, 1H NMR, 13C NMR, and UV. Blending MPHMG, photoinitiator, and double bond-terminated non-ionic water-based polyurethane (NU). After water volatilized, UV-cured the mixture to obtain antibacterial coating PNU. The structure and properties of PNU film were characterized by FTIR, TGA, tensile, water absorption and antibacterial performance tests. The results shown that MPHMG increased the hydrogen bonding in the soft segment and the content of hydrophilic groups of PNU films. When the MPHNG content in the film was 2.0%, the T30% and T50% increased by 7.3 ℃ and 12.6 ℃, the tensile strength increased by 1.6MPa and the water absorption increased by 6.8%. The antibacterial test showed that when the MPHMG content in the film was more than 1.0%, the antibacterial rate against S. aureus and E. coli could reach more than 99.8% after the film exposed to bacteria for 2 hours.