In order to improve the stability of nano-silver antibacterial agent during storage and transportation for prolonging the product validity, the nano-silver solution prepared by using benzalkonium bromide as protective agent and sodium borohydride as reductant was stored under different temperature, UV light irradiation and distinctive initial pH. UV-Vis and dynamic light scattering were used to measure the influence of these environmental factors on nano-silver particle size and Zeta potential. The results indicated the stability of nano-sliver decreased and the micelle trended to aggregation with storage temperature ascending. UV irradiation resulted in larger particle size but smaller absolute values of Zeta potential of nano-sliver solution. Higher initial pH of nano-silver had negative effect on the stability but lower pH benefited for the process. In short, reducing temperature, shielded from light and acidification could improve nano-silver stability. This study would provide reference for selecting appropriate technology parameters and materials during nano-silver manufacturing, transporting and storing.