A semicarbazide derivative R has been synthesized using 3-propyl isocyanate triethoxysilane and 2, 4-Dinitrophenylhydrazine as raw material and characterized by 1H/13C NMR and other methods. The receptor R exhibited excellent recognition capability for F-, Ac- and CN- by obvious changes of UV-Vis spectral and solution color in DMSO solution. In addition, The single recognition effect of the receptor R on CN- can be achieved by adding 30% of the competitive solvent H2O to the DMSO system. According to the Benesi-Hidebrand equation, receptor R recognized CN- with a complex constant of 8.58 ? 103 L/mol and a detection limit of 1.47 uM, and form a 1 : 1 hydrogen bond complex with anions by Job curve. The recognition mechanism of receptor R and anion using hydrogen bonding was explained through 1H NMR titration and theoretical calculation, and no deprotonation behavior was found.