Conjugated microporous polymers (CMPs) are typical porous organic polymers with abundant micro/nanopores and structure tunability. They are widely used in various fields including gas adsorption, selective separation of pollutants and catalysis etc.. However, CMPs synthesized by conventional methods are normally insoluble and unfused with poor processability, which greatly limits their practical applications. In recent years, the construct of CMPs membrane materials with two-dimensional structure has attracted a lot of research attention. The CMPs membrane material not only retains the π-conjugated framework and nano-porous structure of CMPs materials, but possesses excellent machinability of two-dimensional materials. In this paper, the preparation methods of CMPs membrane materials, such as template method and interfacial polymerization, are reviewed. Meanwhile, the application status of CMPs membrane materials in nanofiltration, separation, antibacterial and other fields was also summarized, and the development direction of CMPs membrane material was prospected.