As the membrane material, polyether imide (PEI) was used to prepare PEI flat ultrafiltration membrane by phase inversion method. In order to improve the structure and performance of ultrafiltration membrane, HKUST-1 mixed matrix membrane doped with polyetherimide was prepared by adding metal-organic framework HKUST-1 into the casting solution. The structure, morphology and surface wettability of the prepared film were characterized by FTIR, SEM, AFM and water contact angle tester respectively. It is found that the mixed matrix membrane doped with 0.05% HKUST-1 nanomaterial has good performance. Under the operating pressure of 0.1 MPa, the pure water flux can reach 1304 L/(m2·h), and the rejection rate of 1.0 g/L bovine serum albumin (BSA) is over 97%. After soaking in acidic (pH =2) and alkaline (pH =12) solutions for 24 hours, the rejection rate of BSA only decreased by about 2% (the pure water flux remained basically unchanged), which showed good acid and alkali resistance. At the high temperature of 80℃, the pure water permeation flux of the membrane reached 1587 L/(m2·h) and the BSA rejection rate was still higher than 95%, which showed strong high temperature resistance. Finally, the antifouling performance test showed that its flux recovery rate (FRR) reached 92%, proving that the antifouling performance of the membrane was greatly improved.