In order to develop of the efficient catalytic epoxidation of methyl oleate (MO) macromolecules to prepare the green plasticizer of the epoxidized methyl oleate (EMO). Titanium silicalite-1 (TS-1) and Silicalite-1 (S-1) were synthesized using conventional hydrothermal method, and small sized TS-1 (TS-1-s) and S-1 (S-1-s) catalysts were successfully prepared by steam-assisted crystallization. The effects of sol drying temperature, mass ratio of sol to water, steam-assisted crystallization temperature and crystallization time on the microstructure and catalytic performance of TS-1-s catalyst were investigated. Furthermore, the structure of catalysts was systematically characterized by XRD, UV-Vis, SEM and TEM. The results show that TS-1-s catalyst exhibits better catalytic performance (MO conversion 53.58%, emo selectivity 85.48%) than traditional large grain TS-1 catalyst (MO conversion 39.2%, EMO selectivity 82.56%).