This paper firstly reviewed the research progress of the preparation methods of amphiphilic Janus nanosheets, including interface protection method, emulsion interface self-assembly sol-gel method, template-assisted sol-gel method, and block copolymer self-assembly method. Then, the colloidal properties of amphiphilic Janus nanosheets in aqueous solution and its interfacial properties in oil-water systems were systematically introduced. Finally, the application of amphiphilic Janus nanosheets was briefly introduced, and the future research directions for preparation and colloidal and interfacial properties of amphiphilic Janus nanosheets were looked.