The influence of the microstructure of Mn3O4 on the preparation of mono-like lithium manganate by high temperature solid state reaction. The experimental results show that the precursors have a great influence on the structure and morphology of LiMn2O4. Mono-like lithium manganate, which has dense particle agglomeration, smooth surface, and small cell parameters, high density, and Li-ion molar concentration, is easily made by sphere-like Mn3O4 with small particle size and large specific surface area. The comprehensive electrochemical performanced and thermal stability of the mono-like lithium manganate is excellent and much higher than that of general lithium manganate materials, such as the first discharge specific capacity of 112.50 mAh/g and the coulomb efficiency at 0.2C of 96.5%; the discharge specific capacity is still as high as 102.11 mAh/g at 8C rate, as well as the capacity retention of 90.1% after 200 cycles at 1C. The excellent performance of single crystal lithium manganate is attributed to its more stable crystal structure, larger exposed surface, and higher li-ion molar concentration, but also exhibits higher charge-discharge reversibility, faster li-ion migration rate, lower electrode polarization and charge transfer impedance in electrochemical reactions.