Treatment of industrial dye wastewater with high chromaticity and high toxicity by modified graphene oxide membrane. The graphene oxide (GO) was crosslinked using thiourea (TU). TU-GO nanosheets with m(TU):m(GO)=0.5, reaction temperature 80 °C, reaction time 1h were deposited on polyether sulfone microfiltration membrane (0.22 μm). When the loading capacity was 238.73 mg/m2, the polyether sulfone microfiltration membrane was deposited. The retention rates of methyl orange, Rhodamine B and methylene blue were 94.01%, 87.07% and 99.67%. In order to optimize the separation effect of Rhodamine B, the modified graphene oxide film was prepared by using thiourea (TU) and choline chloride (ChCl) as a eutectic solvent :m(TU):m(ChCl)=2:1, and the modification time was 1 h. The modified graphene oxide film did not affect the original high retention rates of methyl orange and methylene blue, which were 93.95% and 99.24%, respectively. The retention rate of P-Rhodamine B was increased to 99.16%. The reduction mechanism and stability enhancement mechanism of GO during thiourea crosslinking were also analyzed.