Lycopene is a fat-soluble bioactive substance, which is easy to degraded and isomerized during food processing. Natural surfactants were screened, the high cis-lycopene nano emulsion was prepared by high speed shear method using the prepared high cis-lycopene as the embedding object. The preparation process of high cis-lycopene nano emulsion was optimized by single factor and response surface experiments, and the reasonable storage method of high cis-lycopene nano emulsion was explored by stability experiments. The results showed that under the conditions of 1:3 oil-water ratio, 6% sodium caseinate solution concentration, 12 000 r/min shear speed and 15 min shear time, the highest embedding rate of high cy-lycopene nano emulsion was (88.09±0.92) %. The stability experiments showed that high cis-lycopene nano emulsion were stored in low temperature, dark, nitrogen-filled, neutral or weakly alkaline environment. metal ion Al3+, salt ion NaCl, antioxidants (tetra-butylhydroquinone and dibutyl hydroxy toluene) and sucrose were added. The oxidative degradation and retro-isomerization of lycopene isomers could be reduced and inhibited. This scheme can effectively improve the stability and solubility of high cis lycopene nano emulsion, and improve the bioavailability of lycopene.