In order to evaluate the bioactivity of polyphenols from Yunnan Coffee Arabica Pericarp (PYCAP), using purified polyphenols by macroporous resin as raw material, and the inhibitory mechanisms of PYCAP on α-amylase, α-glucosidase were analyzed by Lineweaver-Burk equation and Dixon equation, respectively. The Inhibitory effect of pancreatic lipase and xanthine oxidase were also determined. Finally, the chemical composition was identified by the Ultra Performance Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry (UPLC-QTOF-MS/MS). The results showed that the PYCAP had strong antihyperglycemic, hypolipidemic and uric-acid-lowering effect, and the inhibitory ability exhibited a certain dose effect. The IC50 value of α-amylase and α-glucosidase inhibition were (0.056±0.005) and (0.073±0.002) mg/mL, respectively. Further the inhibition type of α-amylase and α-glucosidase were also competitive-noncompetitive mixed inhibition. In addition, the IC50 value of pancreatic lipase and xanthine oxidase inhibition were (0.034 ± 0.003) and (0.251 ± 0.011) mg/mL, respectively. A total of 27 chemical components were identified, including flavonoids, pyrans, and phenolic acids. These results confirmed the good biological activity of the PYCAP, and provided a scientific foundation for the comprehensive development and utilization of Yunnan Coffee Arabica pericarp.