自粘附动态GA-CS/OKGM多糖水凝胶的制备及性能
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1.陕西科技大学 轻工科学与工程学院;2.中国医学科学院 北京协和医学院生物医学工程研究所

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TQ630

基金项目:

国家重点研发计划“政府间国际科技创新合作”重点专项项目(2022YFE0104200);陕西省科技厅重点研发计划一国际科技合作计划项目(2020KWZ-006)


Preparation and properties of self-adhesive dynamic GA-CS/OKGM hydrogel
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Affiliation:

1.College of Bioresources Chemical and Materials Engineering,Shaanxi University of Science and Technology,Xi&2.amp;3.#39;4.&5.an;6.College of Bioresources Chemical and Materials Engineering,Shaanxi University of Science and Technology,Xi''an;7.Institute of Biomedical Engineering,Chinese Academy of Medical Sciences Peking Union Medical College

Fund Project:

National Key Research and Development Program of China (2022YFE0104200), the International Science & Technology Key.; Research and Development Program of Shaanxi (2020KWZ-006)

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    摘要:

    为制备基于多糖的自粘附、自愈合、可注射水凝胶生物材料,以没食子酸(GA)改性壳聚糖(CS),然后与氧化魔芋葡甘聚糖(OKGM)交联制备多糖水凝胶GA-CSx-OKGM。基于对多糖水凝胶SEM表征和凝胶化时间测定、频率振荡扫描测试、剪切强度测试和降解实验,考察了制备GA-CSx-OKGM过程中,V(CA-CS溶液)∶V(OKGM溶液)对其自愈合、可注射、力学性能和可生物降解性能的影响,并分析最佳力学性能的多糖水凝胶GA-CSx-OKGM的生物相容性,推测其自愈合机理。结果表明,以V(CA-CS溶液)∶V(OKGM溶液)=6∶1制备的GA-CS6-OKGM具有最短的成胶时间(17 s)、最高的储能模量(加载应变1%时为650 Pa)、最低的15 d降解率(68.5%);质量浓度1 mg/mL的GA-CS6-OKGM溶液对大鼠红细胞的溶血率为4.3%,NIH3T3细胞培养实验测得培养24和48 h后的细胞存活率为97%和90%,表现出良好的生物相容性;GA-CS6-OKGM对皮肤、丁腈手套、不锈钢和玻璃都有粘附性。GA-CS和OKGM大分子链上的官能团通过形成动态亚胺键、氢键和静电相互作用赋予水凝胶优异的可注射性、自愈合性和形状适应性,同时,GA中的多酚基团具有与贻贝邻苯二酚基团相似的结构,赋予水凝胶粘附性能。

    Abstract:

    With the continuous development of modern biomedical field, the performance requirements of injectable and self-healing hydrogel are increasing. Improving adhesion property to meet the practical applications while satisfying injectable and self-healing properties is an urgent problem to be solved. Polysaccharide based GA-CS/OKGM hydrogel with injectable, self-healing, and adhesion properties was successfully prepared by modifying chitosan (CS) with gallic acid (GA) and then dynamically crosslinked with oxidized konjac glucomannan (OKGM). The synergistic effect of dynamic imine bonding, hydrogen bonding, and electrostatic interactions in polymer network endows the hydrogel with injectable, self-healing, and shape-adaptability properties. This is structural similiarity between the polyphenol moiety in gallic acid and mussel catechol moiety, endowing adhesive property to the hydrogel. In addition, GA-CS/OKGM hydrogel has excellent biocompatibility, biodegradability, and good mechanical properties, which is a promising smart biomaterial in biomedical fields.

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陈咏梅,杨智炫,杨宽,原汶瑾,张其清.自粘附动态GA-CS/OKGM多糖水凝胶的制备及性能[J].精细化工,2025,42(7):

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  • 收稿日期:2024-05-14
  • 最后修改日期:2024-08-06
  • 录用日期:2024-07-15
  • 在线发布日期: 2025-07-10
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