草莓(Fragaria × ananassa)叶水提物对UVB致 皮肤损伤的保护作用
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Protective effect of strawberry (Fragaria × Ananassa) leaf water extract on skin damage by UVB
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    摘要:

    在草莓叶水提物(Strawberry leaf water extract,SLWE)制备基础上,测定SLWE成分,抗氧化活性、抑制非酶糖基化(NEG)能力,及SLWE对正常生长人皮肤成纤维细胞(HFF-1)细胞活率、透明质酸(HA)、人I型胶原蛋白(COL I)、羟脯氨酸(HYP)、基质金属蛋白酶1(MMP-1)、基质金属蛋白酶9(MMP-9)含量,评价SLWE延缓皮肤衰老功效。在中波紫外线(UVB)致HFF-1损伤模型基础上,测定SLWE对保护组活性氧(ROS)、丙二醛(MDA)、MMP-1和超氧化物歧化酶(SOD)的影响,探讨SLWE对UVB致HFF-1损伤保护机制。结果表明:SLWE富含Vc、槲皮素、绿原酸、没食子酸及儿茶素类物质,其中槲皮素含量12.86 mg/L,表儿茶素含量10.13 mg/L。SLWE自由基清除能力良好,清除不同自由基能力依次为O2-?>DPPH>ABTS>亚铁离子螯和>?OH;水溶液体积分数为1%的SLWE非酶糖基化抑制率为87.17%;体积分数大于3%的SLWE可提高HFF-1中HA、COL I和HYP含量(p<0.05),体积分数为6%的SLWE可降低MMP-1含量(p<0.01),说明SLWE具有提升皮肤含水量,增加胶原含量,抑制胶原降解,实现延缓衰老的功效;在SLWE对UVB致HFF-1损伤保护模型中,SLWE可有效降低因UVB刺激而产生的ROS,体积分数6%的SLWE可显著降MDA,抑制MMP-1表达,提高SOD活力,说明SLWE有助于缓解氧化应激造成的细胞损伤。

    Abstract:

    Based on the preparation of strawberry leaf water extract (SLWE), the composition, antioxidative activities and non-enzymatic glycosylation (NEG) inhibition rate of SLWE was explored. In addition, the effects of SLWE on the survival rate, hyaluronic acid (HA), collagen I (COL I) and hydroxyproline (HYP), matrix metalloproteinase 1 (MMP 1) and matrix metalloproteinase 9 (MMP 9) of normal growing human skin fibroblasts (HFF-1) were evaluated, in order to estimate the effect in delaying skin aging of SLWE. Through building UVB causes HFF-1 damage protection model, the effects of SLWE on reactive oxygen species (ROS), malondialdehyde (MDA), MMP-1 and superoxide dismutase (SOD) in the protection group were measured to explore the protective mechanism of SLWE on UVB-induced HFF-1 damage. The results showed that SLWE was rich in Vc, quercetin, chlorogenic acid, gallic acid and catechin, among which quercetin and epicatechin were 12.86 mg/L and 10.13 mg/L respectively. SLWE had good free radical scavenging ability, which was in order, O2-? > DPPH > ABTS > ferrous chelation > ?OH. The NEG inhibition rate of 1% (volume fraction) SLWE was 87.17%. More than 3% (volume fraction) SLWE could improve contents of HA, COL I and HYP (p < 0.05) in HFF-1, and 6% (volume fraction) SLWE could decrease the content of MMP 1 (p < 0.01), suggesting that SLWE was helpful to anti-aging through lifting the skin moisture, increasing the collagen content and inhibiting collagen degradation. In UVB causes HFF-1 damage protection model, SLWE could effectively ROS generated by UVB stimulation, 6% (volume fraction) SLWE could significantly reduce MDA, inhibit MMP-1 expression, and improve the activity of SOD, indicating that SLWE could help alleviate cell damage caused by oxidative stress.

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董坤,翟文丽,王兰青,何聪芬.草莓(Fragaria × ananassa)叶水提物对UVB致 皮肤损伤的保护作用[J].精细化工,2021,38(4):

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  • 收稿日期:2020-11-01
  • 最后修改日期:2021-01-22
  • 录用日期:2021-01-25
  • 在线发布日期: 2021-03-01
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