Dibutylfuran-2,5-dicarboxylate (DBF) was expected to be a new substitute for petroleum phthalate green plasticizer because of its chemical structure and physical properties similar to phthalate esters, good biocompatibility, and metabolism to human tricarboxylic acid cycle. In this study, the enzymatic synthesis process of bio-based plasticizer dibutylfuran-2,5-dicarboxylate was introduced. In toluene system, dimethyl 2,5-furandicarboxylate was esterified with butanol under the catalysis of lipase, and dibutyl 2,5-dicarboxylate was prepared. The synthesis process of dibutylfuran-2,5-dicarboxylate was optimized, and the effects of sugars and adsorbents as additives on the yield of enzymatic transesterification were discussed. The results showed that when the molar ratio of substrate was 1: 5, the dosage of Novozym435 was 0.005 g/ml, the reaction conditions was 45 ℃,180 r/min in 10 ml toluene solution, and the reaction time was 30 h, the yield of dibutylfuran-2,5-dicarboxylate was the highest. The addition of 4A molecular sieve could obviously promote the formation of dibutylfuran-2,5-dicarboxylate, and the addition of trehalose can also enhance the catalytic ability of lipase. The yield of dibutylfuran-2,5-dicarboxylate reached 87% after adding 0.2 g/ml 4A molecular sieve and 15% trehalose into the reaction system and reacting at 45℃ for 14 h.