文章摘要
郭林新,强涛涛,马养民,任龙芳.响应面法优化马桑皮没食子酸的酶法提取技术[J].精细化工,2021,38(7):0
响应面法优化马桑皮没食子酸的酶法提取技术
Response surface optimization of gallic acid extraction from Coriaria nepalensis bark by biological enzymatic
投稿时间:2020-12-30  修订日期:2021-03-09
DOI:
中文关键词: 响应面  单宁酶  马桑皮  没食子酸  提取技术
英文关键词: response surface  tannase  Coriaria nepalensis bark  gallic acid  extract technology
基金项目:陕西省重点科技创新团队(2020TD-009);陕西省教育厅重点科学研究计划(协同创新中心项目)(20JY003)
作者单位E-mail
郭林新 陕西科技大学轻工科学与工程学院 glx9933@foxmail.com 
强涛涛 陕西科技大学轻工科学与工程学院 qiangtt515@163.com 
马养民 中国轻工业轻化工助剂重点实验室  
任龙芳 陕西科技大学轻工科学与工程学院  
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中文摘要:
      采用单宁酶法从马桑皮中提取没食子酸,以单因素-响应面实验优化了提取工艺,对得到的没食子酸进行了纯化和结构鉴定,考察了纯化后没食子酸的抗氧化活性,并与传统的酸提取法相对比。实验结果表明,在以马桑皮原料用量为基准,单宁酶的添加量为20 u/g、体系pH 5、提取温度48 ℃、提取时间4 h的条件下,没食子酸提取率可达10.79%±0.65%。经NKA-9大孔树脂纯化后,酶法制备没食子酸的纯度为96.0%,高于酸提取法制备的没食子酸纯度81.3%,其结构经1HNMR、13CNMR及异核单量子关系(HSQC)进行表征。相比于酸提取法,酶法制备的没食子酸具有更强的抗氧化活性,在质量浓度为256 mg/L时,其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟基自由基清除率分别为78.81%和61.99%,对Fe3+还原能力值为0.685。
英文摘要:
      Tannase was used to extract gallic acid from Coriaria nepalensis bark, and the extraction process was optimized by single factor and response surface experiments. The gallic acid extraction was purified and identified, and the antioxidant activity of the purified gallic acid was investigated and compared with the result of traditional acid extraction method. The experimental results show that when the amount of Coriaria nepalensis bark is used as the benchmark, the extraction yield of gallic acid can reach to10.79%±0.65% under the conditions of 20 u/g tannase addition in extraction pH 5 at 48 ℃ over 4 h. After purification by NKA-9 macroporous resin, the purity of gallic acid prepared by enzymatic method is 96.0%, which is higher than the purity of 81.3% prepared by acid extraction method. Its structure was characterized by 1HNMR, 13CNMR and heteronuclear singular quantum correlation (HSQC). Compared with acid extraction method, gallic acid prepared by enzymatic method has stronger antioxidant activity. When the mass concentration was 256 mg/L, the scavenging rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and hydroxyl radicals were 78.81% and 61.99%, respectively, and the reduction ability value of Fe3+ was 0.685.
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