Fructosazine was synthesized from D-gluctose by 4 steps，including Amadori rearrangement, isopropylidene protection, cascade reaction of hydrodebenzylation and oxidative aromatization catalyzed by Pd/C, and removal of isopropylidene protection. The structure of the key intermediate isopropylidene fructosazine was characterized by NMR, MS, X-ray single crystal diffraction. The MTT method was used to determine the effect of fructosazine on the viability of human fibroblast cells (2BS cells). Compared with the control group, 2BS cell viability was significantly enhanced 25% by 1 μmol/L fructosazine. The aging marker β-galactosidase (SA-β-Gal) method was used to evaluate the anti-aging activity of fructosazine. The blue staining rate of early-senescent and mid-senescent 2BS cells, which were continuously cultured with 1 μmol/Lfructosine, were 14% and 19% less than that of the control group respectively. The results showed that fructosazine had anti-aging activity on 2BS cells in the early or middle stage of aging.