The antioxidant activity of the extracts of Cultivate Cistanche deserticola was assayed by the method of DPPH, ABTS and FRAP in vitro. Mouse model of acute hepatic injury was established by giving CCl 4 abdominally. The activities of ALT and AST in serum and the levels of MDA and SOD in liver tissue were examined to evaluate the hepatoprotective and antioxidant activity of ethanol extract of Cultivate C. deserticola. The ethanol extract was the highest antioxidant activity in four extracts which the values of RACT50 were 3534.32 祄ol/g, 2726.39 祄ol/g and 2621.68 祄ol/g in DPPH, ABTS and FRAP assay respectively. It was slightly lower than that of BHA as positive control, and higher than that of BHT. The ethanol extract of Cultivate C. deserticola significantly reduced the activities of ALT and AST in serum and the levels of MDA in liver tissue, and significantly raised the levels of SOD in liver tissue. The results showed that the ethanol extract of Cultivate C. deserticola had a significant hepatoprotective effect in CCl4-induce acute liver injury. The mechanisms may be related to its effects of reducing lipid peroxidation product.