To investigate the yeast and rat intestinal α-glucosidase inhibitory activity of Lindera glauca (Sieb1et Zucc) Blume. in vitro and vivo. Its α-glucosidase inhibitory activity was assayed by the method of 96-microplates. The results showed that yeast α-glucosidase inhibitory activity of petroleum ether extracts (IC50 = 229.70 μg/mL), ethyl acetate extracts (IC50 =259.10 μg/mL) and n-butanol extracts was lower than that of Acarbose (IC50=1081.27 μg/mL) as positive control, and only ethyl acetate extracts (IC50 =418.17 μg/mL) showed rat intestinal α-glucosidase inhibitory activity. The IC50 value of Acarbose did not be detected. The results indicate that all the extracts of L. glauca showed a better yeast α-glucosidase inhibitory activity and only ethyl acetate extracts showed a better rat intestinal α-glucosidase inhibitory activity.