The synthesis of trimethylolpropane trisalicylate was implemented.Under the protection of nitrogen,trimethyl-olpropane trisalicylate was synthesized by direct este-rification process when trimethylolpropane and salicyl- ic acid was used as reactants, the product was analyzed by IR、1H-NMR、MS. The structure of the target product was confirmed. The influencing factors, such as tempe- rature, catalyst, molar ratio of salicylic acid to tri-methyololpropane and reaction time were studied.The op- timum reaction conditions were obtained as follows: tosic acid as catalyst, molar ratio of the carboxyl of salicylic acid to the hydroxy of trimethyolpropane 1:0.7, reacting in N2 for 6 h at 160 ℃. Under the optimum con- ditions the yield of the product reached 99.5 %. The anti-bacterial properties of the esters were studied, the results showed that: the trimethylolpropane trisalicylate has the very obvious inhibitory effects to Escherichia coli.