Five strains of Endophyte were purified from the endophyte in fresh lichees, One of them was selected as a sample because of its fragrant metabolite and speedy growth. The metabolite which was analyzed by GC-MS showed that the fragrance components were mainly from β-phenylethyl alcohol, 2,3-butanediol, furfural and n-hexadecanoic acid. The product of fermented tobacco flavor Lizhi-A from this kind of the sample strain had ability to make smoke more gentle, smooth and tobacco tasted gently, tenderly and rich.