N-(3-chlorine-2-hydroxypropyl)-N,N-dimethyl-N-dodecyl-ammonium chloride (CHPDDAC) was synthesized with N,N-dimethyl dodecyl amine hydrochlorate and epichlorohydrin. Then, Alkyl polyglucosides(APG) was quaternizing modified into Alkyl polyglucosides quaternary Ammonium Salt(APGQAS) by CHPDDAC. The effects on the reaction process were investigated by the orthogonal experiment, The optimum conditions of the APGQAS were obtained as follows: isopropyl alcohol as solvent; n(APG):n(CHPDDAC) = 1.1:1; reaction temperature 80℃; reaction time 7h. The structure of APGQAS was characterized by FTIR spectrum, elementary analysis and 1HNMR. The surface activities of the product were measured, the critical micelle concentration(CMC) is 3.16×10-4mol/L, surface tension is 21.51mN/m, Krafft point is -3.57℃.