In present study, the characteristics of eighteen macroporous resins for the static adsorption and desorption of hypericin from Hypericum perforatum L.extracts have been evaluated. And then the effect of different pH values of extract solution on the adsorption capacities of hypericin on NKA-9 resin has been explored. The Langmuir isotherm was used to describe the interactions between solutes and resins at 25℃. The results showed that a week polar resin, NKA-9, with the adsorption capacity of 12.45 mg/g (dry resin) offered the higher adsorption and desorption capacity for hypericin than other resins. The pH had a great effect on the adsorption process, and the optimum pH was 4. The adsorption process of NKA-9 at 25℃fit best to the Langmuir isotherm, proved to be single molecule adsorption.