The volatile compounds were extracted in the different parts of Humulus scandens (Lour.)Merr. by microwave-assisted headspace solid-phase micro-extraction(MAE-HS-SPME). Their constituents were analyzed and identified by gas chromatography-mass spectrometry combined with Kovats retention index, and were quantified by calculating the relative contents basing on the peak areas normalization method. The results showed that 86 volatile constituents in the stem, 90 in the leaf and 103 in the flower of Humulus scandens (Lour.)Merr. were separated and identified. Accounting for 96.690 %、96.443% and 94.097% of the total peak areas, respectively. Which is the main component in stem carvacrol (5.702%), thymol(9.407%),β-caryophyllene(6.884 %) ,α- bergamotene (5.511%),trans-β-farnesene(5.965%),1,3-dimethylbicyclo[3.3.0]oct-3-en-2-one(14.010%) and β-bisabolene (5.798%). And in the leaves β-caryophyllene (12.704%),α-bergamotene (8.588%),trans-β-farnesene (6.662%) and 1,3-dimethylbicyclo[3.3.0]oct-3-en-2-one (7.487%). And in the flowers β- pinene (4.719%), β-caryophyllene (8.201%),trans-β-farnesene(11.906%) and α- humulene(5.515%). It was also observed that there were significant differences in the relative contents of three parts.