On the base of using sodium tripolyphosphate(TPP) as crosslinker, tuberose fragrance-loaded chitosan nanoparticles were prepared by ionic gelation method. The effects of the mass concentration of chitosan、the mass concentration of tuberose fragrance、the mass ratio between emulsifier and fragrance and the reaction time to the size distribution of tuberose fragrance-loaded chitosan nanoparticles were studied. The tuberose fragrance-loaded chitosan nanoparticles were characterized by dynamic light scattering(DLS)、transmission electron microscopy(TEM)、infrared spectroscopy(IR) and thermogravimetric analysis(TGA). Results showed that the average particle size was 138nm, the polydispersity was 0.100 and the fragrance loading capacity was 28.4% when the chitosan mass concentration was 1.71g/L、the fragrance mass concentration was 1.72g/L、m(emulsifier):m(fragrance)=1:2 and reaction time was 1h. Under high temperature, the release rate of tuberose fragrance loaded by chitosan nanoparticles was decreased.