Abstract: In order to gain the optimization extraction condition of isoflavones from Fructus Sophorae by microwave-assisted techniques , we have inspected single factor influence and then optimized them by orthogonal experiment. The results of orthogonal experiment indicated that the yield of Fructus Sophorae isoflavones in the extracts were 52.43% and the purity w(Fructus Sophorae isoflavones)of the products was 36.78% when the liquid-solid ratio of 12.5mL•g-1 at 500 W microwave power at 60 ℃ degree for 7 min.