Abstract: MxOy-V2O5/Al2O3 catalysts were prepared by the impregnation method，at the same time,in order to investigate the MoO3-V2O5/Al2O3 catalytic performance, the MoO3-V2O5/Al2O3 was prepared by the different methods. The surface area, redox properties and the oxygen desorption behavior of catalysts were characterized by BET,H2-TPR and TPD-MS. The analysis of the used catalysts showed that the MoO3-V2O5/Al2O3 , Fe2O3 -V2O5/Al2O3 and Cr2O3 -V2O5/Al2O3 presented the higher number of surface oxygen and conversion rates of α-pinene, the more O2- and O- species exist on the surface of MoO3-V2O5/Al2O3 catalyst, the O2- and O- species can increase catalytic activity and yield of myrtenal. The different pre paration methods would influence on the catalytic performance of MoO3-V2O5/Al2O3 catalysts, the surface modification method could come into being more O2- and O- species，which is propitious to improve catalytic activity, the conversion rates of α-pinene is 88.9%,the yield of myrtenal is 79.1%.