Allyl polyoxyethylene glycidol ether(APGE) was synthesized with allyl polyoxyethylene ether, epichlorohydrin and sodium hydroxide solution as raw material through two-step method. Polyoxyethylene glycidol ether trisiloxane (PGETS) was prepared by the hydrosilylation from 1,1,1,3,5,5,5-heptamethyltrisiloxane(MDHM) and APGE; and then further underwent a ring-opening reaction with trimethylamine hydrochlorate to form quaternary ammonium salt polyoxyethylene ether trisiloxane surfactant (QASPETSS). The chemical structure of QASPETSS was characterized by IR and 1HNMR. The interfacial properties of QASPETSS were studied by the measurement of equilibrium surface tension in its aqueous solution. Experimental results showed as follows: In the critical micelle concentration of 6.3×10-4mol•L-1, the surface tension of QASPETSS aqueous solution reduced to approximately 22.4 mN•m-1. The saturated adsorption, single QASPETSS average areas on the saturated absorption layer and the micellization standard free energies are 3.6×10-6mol•m-2, 0.46 nm2 and -28.2 kJ•mol-1, respectively.