Supercritical CO2 anti-solvent technology was tested to prepare zein nanoparticles loaded with resveratrol. The effects of different operating parameters, such as pressure, temperature, and ratio on the loading weight of resveratrol were investigated UV, SEM, XRD were used to characterize the products. The release behavior of resveratrol from the prepared nanoparticles was also studied. Results show that the yield of resveratrol is lower when increasing CO2 pressure. However, while temperature and ratio are increased, the loading yield is higher The structure of resveratrol-loaded zein nanoparticle is a Matrix with good sphere shape。Furthermore, in vitro drug release studies show that products exhibit a slower release than the single resveratrol.