Triglycol benzo[5,6]coumarin-3-carboxylate (D) was prepared and characterized by 1H NMR, 13C NMR, FT-IR, MS and elemental analysis. Compound D and triglycol dibenzo[5,6]coumarin-3-carboxylate (E) emit blue fluorescence. The maximum emission wavelength λem of D and E was little influenced by solvent polarity. The fluorescence intensity of E was increased in methanol and ethanol, and dimethyl sulfoxide is its fluorescence quenching agent. In acetonitrile, upon the addition of Ca2+, the fluorescence intensity of D reduced by 30 %, while that of E increased by 40 %.