The three different Ni-Cu/γ-Al2O3 bimetallic catalysts were prepared by co-precipitation method, impregnation method and co-precipitation - impregnation method. Under different conditions, the three Ni-Cu/ γ-Al2O3 catalysts for dehydrogenation of the hydrogen carrier catalytic performance were investigated by using a continuous tubular micro-reactor. XRD, SEM, BET and BJH were used to characterize the catalysts. The results showed that: Using Ni-Cu/γ-Al2O3 catalyst prepared by co-precipitation - impregnation method is better than the others (the Ni-Cu loading of the three catalysts were 26% and the proportion of nickel and copper was 8 to 1). Under the conditions of the reaction temperature 673K, reaction pressure 0.6Mpa, MCH/N2 = 19/27 and the volume of gas space velocity 240 h-1 , MCH dehydrogenation conversion rate reached 98.5% and the product selectivity was close to 100% .Besides, it is feasible that using the Ni-Cu/γ-Al2O3 bimetallic catalyst is better than the platinum or other noble metal catalysts and nickel catalysts in this reaction system.