N,N’-di-allyl-dithiodipropionamide(ALPD)has been synthesized with allylamine and dithiodipropionmethyl by amidation , and its structure has been confirmed by IR spectroscopy and 1HNMR. Considering molar ratio of the reactants, amount of the catalyst, dropping time of dithiodipropionmethyl and reaction time impact on the yield of ALPD; researching the antibacterial effects of ALPD. The optimum reaction conditions are n（allylamine）: n（dithiodipropionmethyl）=3:1, the amount of catalyst triethylamin is 2.5mL, dropping time of dithiodipropionmethyl is 2.5h, reaction time is 48h, yield is 88.4%. The MICs of ALPD are 0.1250g/L, 0.0625 g/L with Staphylococcus aureus, Escherichia coli. The ALPD can be introduced into the macromolecular by polymerization.