The essential oil was extracted from Trollius chinensis Bunge by steam distillation extraction, simultaneous distillation extraction, ultrasonic extraction, homogenate extraction simultaneous distillation extraction followed ultrasonic extraction, simultaneous distillation extraction followed homogenate extraction methods. The constituents of essential oil were isolated and identified by GC-MS, and the applications of the oil in unblended cigarette flavoring were studied as well. The results showed that: 1) The extraction rate of oil extracted by the six methods was 0.20%, 0.67%, 2.31%, 2.67%, 0.82%, 0.78% and 20, 30, 19, 18, 37, 30 compounds were identified, respectively; the primary chemical components were β-ionone and dihydroactinidiolide, etc; among of them, the total amount of essential extracted by ultrasonic extraction, homogenate extraction are higher relatively, but they more impurity, the oil extracted by simultaneous distillation extraction followed ultrasonic extraction is better than the others. 2) It could play an important role in enhancing the aroma quality of cigarette, obviously reducing the irritation, and smoothing the smoke while essential oil of simultaneous distillation extraction followed ultrasonic extraction is better than the others.