For the reaction system of cyclohexene oxidation product epoxy cyclohexane by hydrogen peroxide, Using the technology of macroporous resin to adsorption and separation 1,2-Cyclohexanediol in reaction aqueous solutions. In basis of the self-made absorption column, the results showed that the better conditions for adsorption and separation 1,2-cyclohexanediol in reaction aqueous solutions by AB-8 resin were: sample flow rate 1.0 mL/min, bed height 4.0 cm, normal temperature. The rate constants, correlation coefficients, adsorption capacity and kinetic parameters, which were calculated through mathematical models, can be used to describe the adsorption kinetics of 1,2-Cyclohexanediol by AB-8 resin well. The better conditions of desorption using ethyl acetate were: elute rate 1.0 mL/min, normal temperature. The absorption rate and desorption rate of AB-8 resin can still reach persantage of 80 after recyling five loops.