In this paper, a compound of 5-bromo-2-hydroxyphenyl methyl ketone-4-nitrobenzoyl hydrazone (H2L) was synthesized through the reaction of 5-bromo-2-hydroxyphenyl ethyl ketone with 4-nitrobenzoyl hydrazine and ethanol. A complex of new binuclear 5-bromo-2-hydroxyphenyl (methyl) methylene-4-nitrobenzohydrazide pyridinezinc(II) (CCDC:851546) was hydrothermal synthesized through the reaction of the ligand with pyridine and zinc nitrate, and was subsequently into a single crystal. The compound was characterized by elemental analysis, IR and the crystal structure was determined by X-ray single crystal diffraction. The results show that the crystal belongs to the monoclinic system with space group P21/c, with a=1.17989(9) nm, b=1.64805(12) nm, c=1.02300(8) nm, β= 96.9670(10)º, Dc=1.751 mg/m3. Fluorescence spectra show that both the ligand and complex have fluorescent properties, the fluorescence intensity of complex has been significantly enhanced in 475.0 nm.