Three dithiodipropionamide derivatives , N’N-dimethoxypropyl- dithiodip- ropionamide（MPD）,N’N-dibutoxypropyl-dithiodipropionamide (BPD),N’N-dinoctyl- dithiodipropionamide（OLPD）were synthesized from methyl acrylate in the aqueous phase and detected by infrared spectrometer and NNR spectrometer. The bioassay results showed that all the three compounds possessed good antibacterial activities, with MIC value of BPD to Escherichia Coli and Staphylococcus aureus reached 0.0625 mg/mL.Then the compounds were put into paintings made them antifouling paintings. The study of their antifouling properties by submerging in the ocean showed that they all have good qualities of antifouling. The BPD showed the best antifouling property.