Six fluorinated 1,3,4-thiadiazolylureas were synthesized by reaction of fluorine-containing benzoyl azides with 2-amino-5-(fluorine-containing styryl)-1,3,4-thiadiazoles, which were prepared in one step through the condensation-cyclization of fluorine-containing cinnamic acid and thiosemicarbazide with POCl3 as dehydrating agent, in the yield of 68%, 69%, 66%, 67%, 71% and 73% respectively. The structures of the products were confirmed by IR, 1H NMR, MS and elemental analysis. The preliminary bioassay showed that target compounds IIIc and IIId exhibit good cytokinin activity at the concentration of 10 mg/L, and the promoting rates of them are 53.5% and 55.1%, respectively.