The β-cyclodextrin microspheres were synthesized in inverse emulsion with β-cyclodextrin as material and epichlorohydrin as cross-linking agent, then the rose perfume was cured by them. The preparation process was optimized through the L9(34) orthogonal experimental design and the structure and performance were identified by FT-IR spectroscopy, X-ray diffraction (XRD) and thermogravimetric analysis (TGA). The extraction rate of rose perfume and the yield of inclusion compound can reach 64.2% and 89.5% respectively under the following conditions : m(β-CD microsphere/g):V(perfume/mL)=4:1, time 4h, emperature 50℃, stirring 600 r•min-1. Influence factors were the percentage of the rose perfume oil in β-CD microsphere inclusion, the reaction temperature, the agitating rate, the reaction time in order.