A series of novel quaternary amphiphilic chitosan derivatives(DEAE-CMC)were prepared.The DEAE-CMC/VB12 microspheres were prepared by “Emulsion—Crosslinking—Solidification” method. The partical size, topography and microstructure of the microspheres were analyzed by laser particle size analyzer and scanning electron microscopy (SEM).The result showed that the average partical size of the DEAE-CMC/VB12 microspheres was 4.53 μm。The drug release behavior of the DEAE-CMC/VB12 microspheres in pH=7.4 phosphate buffer solutions showed that: the release equilibrium time was 60 h, the entrapment efficiency was 33.70 ,and the loading content was 12.47 %. At the equilibrium, the accumulated release ratio of the drug was 56.30 %.