Nonylphenol polyoxypropylene polyoxyethylene oxide sulfonates (NPESO-3-4) was synthesized and content of raw materials in product was analyzed by high-performance liquid chromatography (HPLC). Surface tension, emulsifying ability, wettability and mimic oil recovery efficiency of NPESO-3-4 were measured and also compared with Nonylphenol polyoxyethylene oxide sulfonates’ (NPESO-0-4). The results showed that for surfactants in aqueous solution, the NPESO-3-4 caused a large increase in the effectiveness of surface tension reduction, while its efficiency was slightly worse than NPESO-0-4. The interfacial tensions between NPESO-3-4 and NPESO-0-4 aqueous solution/Shengli crude oil can reach the range of 10-1 mN?m-1. NPESO-3-4 could alter wettability of the surface from oil-wet to intermediate wet, but emulsifying ability and mimic oil recovery efficiency for NPESO-3-4 was significantly higher than NPESO-0-4, which indicated the Anionic-Nonionic surfactants with polyoxypropylene group were suitable for Enhanced Oil Recovery.