On the basis of the stability constant determination of zingiberene-Ag+ coordination compound, zingiberene static adsorption on silver nitrate modified silica gel was compared with that on silica gel , and the thin layer chromatography of Ginger oleoresin on argentated silica gel G pre-coated plate with that on silica gel G pre-coated plate, finally modified silica gel column chromatography was used to isolate zingiberene from ginger oleoresin．The comparison results indicated that zingiberene static adsorption was weak when silica gel was treated as medium, and the adsorption capacity was enhanced after silica gel was modified with silver nitrate, whenever the adsorption conforms to the Langmuir monomolecular adsorption. Zingiberene-Ag+ π coordination compound could be formed with the stability constant was 1.46×10-2L•mol-1when silver nitrate was added．The stability of sesquiterpene-Ag+ coordination compounds is related to their structure and the solvents, consequently when silica gel was modified with silver nitrate, the selective adsorption of zingiberene was increased, zingiberene was effectively separated from Ginger oleoresin and the purity of the final products was 97.22% with 87.15% recovery.