Acetone-Butanol-Ethanol (ABE) fermentation continuously coupling with pervaporation was conducted in a continuous and closed-circulating fermentation system by combining clostridium acetobutylicum and PDMS membrane bioreactor. The continuous fermentation was successfully lasted for 192 h, and the corresponding kinetic behaviors were studied. During the continuous fermentation, cell concentrations of 0.84~4.00 g/L, glucose concentrations of 16.08~35.15 g/L, the total solvents concentrations of 5.14~17.54 g/L in the fermentation broth and ABE productivity of 0.36 g/(L?h) were achieved. The results showed that ABE fermentation continuously coupling with pervaporation in a PDMS membrane bioreactor system is feasible and promising.