Influence of reduction temperature on the reduction degree and performance of CuO-ZnO-ZrO2 catalyst for carbon monoxide oxidation at low temperature is investigated by means of reduction thermodynamic calculation, H2-TIR, H2-TPR and catalytic tests. It is found that the introduction of ZrO2 to CuO-ZnO improves the dispersion of active sites CuO with easy redox capacity and the transformation of valence state from Cu2 to Cu1 is easier than to Cu0. H2-TIR results indicate that the H2 consumption during reduction process increases, and the reduction peaks move forward with the increase of reduction temperature from 100℃ to 200℃. From the calculations by method of H2 consumption, the reduction degree of catalyst is found to increase with reduction temperature, in the order of 0% (100℃) < 45.7% (140℃) < 77.3% (160℃) < 86.2% (200℃). The catalyst activated at the optimal reduction temperature of 160℃ exhibits good catalytic performance, that shows 100% CO conversion in CO-probe oxidation at 100℃,and 0.02 ppm CO removal depth in the application of CO removal from liquid propylene.