Poly(amine-ester)-lauric acid dendrimer (PAE-LA) is synthesized from ethylenediamine (EDA), trimethylolpropane triacrylate (TMPTA) and Lauroyl chloride, the yield is 56.09%. The structure of PAE-LA is charactracterized by IR and 1HNMR. The effects of PAE-LA concentraction and water phase pH on the phase transfer rates of dyses(such as methyl orange, titan yellow and xylenol orange) were investigated. The results show that the phase transfer rate of dyes increase with the increase of the concentraction of PAE-LA. In the mixture with 20 mL PAE-LA- chloroform solution1.2 ?0-5 mol/L (c))，and 20 mL dye(methyl orange, titan yellow ,or xylenol orange) water solution(about1.200?0-5 mol/L(c)) ,when water phase pH is 2, the phase transfer rates of titan yellow, methyl orange, xylenol orange reach the maximum (83.75%、81.96%、57.35%) respectively.