By dispersing nano-particle directly to get nano-TiO2/WPU composite membrane, meanwhile, we observed the affect of performance which added to crystal nano-TiO2(rutile and anatase type) on the composite membrane. The analysis of viscosity showed the rutile type had better effects on the increase of viscosity. The analyses of grain diameter showed that there were no differences on the stability of polyurethane-emulsion whether adding nano-TiO2 or not, and the composite particle still existed as the state of nanoscale, still, both type of rutile and anatase nano-TiO2 dispersed uniformly in composite film and had no effects on the flatness of it. The contacting angle and hygiene performance tests indicated that both crystal-size of composite film had no water-proof properties, the one which added anatase type had better hygiene function; the mechanical and anti-bacterial tests manifested the one added anatase type had better mechanical and anti-bacterial properties.