The functional effect factors (conjugation, heavy-atom or steric effect) were used to design and synthesis four Cu(II) complexes based on Schiff-base Cu(II) complexes 1 (Cu(II) with N,N’-bis (3-methoxy-salicylidene)ethlene-1,2-diamine) and complexes 4 (Cu(II) with N,N’-bis(3-methoxy-salicylidene)phenylene-1,2-diamine). The structure of complex 2 was characterized by X-ray diffraction analysis. Furthermore, using NBT illumination method to determine those anti-oxidant activities, the result shows that, after changing structure, the anti-oxidation activity of complexes has been improved significantly. Additionally, several factors definitely influence the activity but the degree of influence were different.