In this paper, two Schiff bases（a and b） of acetylferrocene and two heterocyclic amines(4-aminoantipyrine,2-amino-5-mercapto-1,3,4–thiadiazole)have been synthesized,the rate of production was 85.8% and 72.6% respectively.The structures of these two compounds were characterized by IR，1H NMR and elemental analysis. Finally, the primary antibacterial activities have been screened for these two compounds against three bacterias(Staphylococcus aureus, Escherichia coli, Bacillus subtilis)，the result were compared with the antibacterial activity of non- heterocyclic Schiff base(c). Finally, the result indicate that Schiff bases exhibited antibacterial activities against these three bacterias, and bacteriostatic effect along with the increase of the Schiff bases concentration and enhancement.In addition, heterocyclic Schiff bases (a, b ) against on the above three kinds of bacteria bacteriostatic activity are obviously superior to the non-heterocyclic Schiff base (c).