Abstract: Tuberose fragrance loaded monochlorotriazinyl-β-cyclodextrin(MCT-β-CD) nanoparticles were prepared by the inclusion complexation method. The effects of the volume ratio between athenol and water, the mass ratio between tuberose and MCT-β-CD, the reaction time , the stirring rate and the reaction temperature to the size distribution of tuberose fragrance-loaded MCT-β-CD nanoparticles were investigated. The tuberose fragrance-loaded MCT-β-CD were characterized by dynamic light scattering(DLS), Transmission electron microscope( TEM ), Infrared Spectrometer ( IR ) and Thermogravimetric Analyzer ( TGA ). Results showed that the particle size was 216.4 nm, the polydispersity index(PDI) was 0.221 and the fragrance loading capacity was 17.2% when v(CH3CH2OH): v(H2O) = 1:0.7, m(fragrance): m(Wall Materials) = 1:15, reaction time was 10 h, rotational speed was 1300rpm and reaction temperature was 35℃. The release rate of tuberose fragrance-loaded MCT-β-CD nanoparticles was decreased under the high temperature.