Amplification experiments of preparing dodecyl glycoside in the 30ml reactor were founded in the lab. Effects of technological parameters on preparing stability were investigated. The acceptable product could not be gained according to the experimental conditions of small scale plant. Water content in the glucose, raw material ratio, dispersity in the raw material, and the stability of the technological parameters were all the main influence factors of amplification experiments. Experiments indicated that the best technological parameters were as follows. Glucose with no water must be sieved, premixed and batch fed. Raw material ratio was equal to 6:1. Temperature, pressure and so on, were automatically controlled by computer. Condenser and knockout drum were added to the vacuum plant. The accuracy and reliability of the method have been verified.